Yazar "Eroğlu, Emrah" için listeleme
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Active shrinkage protects neurons following axonal transection
Aydın, Mehmet Şerif; Bay, Sadık; Yiğit, Esra Nur; Özgül, Cemil; Kaval Oğuz, Elif; Yenidünya Konuk, Elçin; Ayşit, Neşe; Cengiz, Nureddin; Erdoğan, Ender; Him, Aydın; Koçak, Mehmet; Eroğlu, Emrah; Öztürk, Gürkan (Elsevier Inc., 2023)Trauma, vascular events, or neurodegenerative processes can lead to axonal injury and eventual transection (axotomy). Neurons can survive axotomy, yet the underlying mechanisms are not fully understood. Excessive water ... -
Chemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors
Ghaffari Zaki, Asal; Erdoğan, Yusuf Ceyhun; Akgül Çağlar, Tuba; Eroğlu, Emrah (Portland Press Ltd, 2022)Chemogenetic tools are recombinant enzymes that can be targeted to specific organelles and tissues. The provision or removal of the enzyme substrate permits control of its biochemical activities. Yeast-derived enzyme D-amino ... -
Chemogenetic tools and genetic biosensors in redox biology: Probing Hyper7 signals in specific cellular locales
Altun, Hamza Yusuf; Erdoğan, Yusuf Ceyhun; Seçilmiş, Melike; Eroğlu, Emrah (Elsevier Science Inc., 2023)H2O2 is one of the most studied reactive oxygen species (ROS) in redox biology because of its critical role in oxidant-dependent signaling pathways. Conventional approaches apply exogenous H2O2 on cells and tissues, which ... -
A co-culture-based multiparametric imaging technique to dissect local H2O2 signals with targeted HyPer7
Seçilmiş, Melike; Altun, Hamza Yusuf; Pilic, Johannes; Erdoğan, Yusuf Ceyhun; Çokluk, Zeynep; Ata, Büşra Nur; Sevimli, Gülşah; Zaki, Asal Ghaffari; Yiğit, Esra Nur; Öztürk, Gürkan; Malli, Roland; Eroğlu, Emrah (MDPI, 2021)Multispectral live-cell imaging is an informative approach that permits detecting biological processes simultaneously in the spatial and temporal domain by exploiting spectrally distinct biosensors. However, the combination ... -
Complexities of the chemogenetic toolkit: Differential mDAAO activation by D-amino substrates and subcellular targeting
Erdoğan, Yusuf Can; Altun, Hamza Yusuf; Seçilmiş, Melike; Ata, Büşra N.; Sevimli, Gülşah; Çokluk, Zeynep; Zaki, Asal Ghaffari; Sezen, Serap; Akgül Çağlar, Tuba; Sevgen, İlker; Steinhorn, Benjamin; Ai, Huiwang; Öztürk, Gürkan; Belousov, Vsevelod V.; Michel, Thomas; Eroğlu, Emrah (Elsevier Inc., 2021)A common approach to investigate oxidant-regulated intracellular pathways is to add exogenous H2O2 to living cells or tissues. However, the addition of H2O2 to the culture medium of cells or tissues approach does not ... -
Development of a chemogenetic approach to manipulate intracellular pH
Zaki, Asal Ghaffari; Miri, Seyed Mohammad; Çimen, Şeyma; Akgül Çağlar, Tuba; Yiğit, Esra Nur; Aydın, Mehmet S.; Öztürk, Gürkan; Eroğlu, Emrah (American Chemical Society, 2023)Chemogenetic Operation of iNTRacellular prOton Levels(pH-Control)is a novel substrate-based enzymatic method that enables precise spatiotemporalcontrol of ultralocal acidification in cultured cell lines and primaryneurons. ... -
Importance of physiological oxygen levels for endothelial redox signaling using Hyper 7
Altun, Hamza Yusuf; Yang, Fan; Mann, Giovanni; Eroğlu, Emrah (Elsevier Science Inc, 2023)Cellular redox status is tightly linked to ambient oxygen levels. Endothelial cells (EC) in vivo are exposed to O2 levels between 3- 13 kPa, yet the majority in vitro studies maintain EC under atmospheric O2 levels (~18 ... -
Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels
Sevimli, Gülşah; Smith, Matthew J.; Akgül Çağlar, Tuba; Bilir, Şükriye; Seçilmiş, Melike; Altun, Hamza Y.; Yiğit, Esra N.; Yang, Fan; Keeley, Thomas P.; Malli, Roland; Öztürk, Gürkan; Mann, Giovanni E.; Eroğlu, Emrah (Elsevier B.V., 2022)Iron is an essential metal for cellular metabolism and signaling, but it has adverse effects in excess. The physiological consequences of iron deficiency are well established, yet the relationship between iron supplementation ... -
Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels (vol 53, 102319, 2022)
Sevimli, Gülşah; Smith, Matthew J.; Akgül Çağlar, Tuba; Bilir, Şükriye; Seçilmiş, Melike; Altun, Hamza Y.; Yiğit, Esra N.; Yang, Fan; Keeley, Thomas P.; Malli, Roland; Öztürk, Gürkan; Mann, Giovanni E.; Eroğlu, Emrah (Elsevier, 2022)The authors regret that the cited reference list in the online PDF of this article contains a duplicate reference (Ref 34 and incomplete Ref 56). Ref 56 should read as follows. -
The preamble to the free radical biology and medicine virtual special issue on “Targeting genetic biosensors to intracellular signaling pathways”
Eroğlu, Emrah; Graier, Wolfgang F.; Malli, Roland (Elsevier Inc., 2022)Studying biological signaling pathways is key to understanding the various processes within a cell. The science of biological redox regulation is a rapidly growing research area with implications for various disciplines, ... -
Probing subcellular iron availability with genetically encoded nitric oxide biosensors
Sevimli, Gülşah; Alston, Amy E.; Funk, Felix; Flühmann, Beat; Malli, Roland; Graier, Wolfgang F.; Eroğlu, Emrah (MDPI (Multidisipliner Digital Publishing Institute), 2022)Cellular iron supply is required for various biochemical processes. Measuring bioavailable iron in cells aids in obtaining a better understanding of its biochemical activities but is technically challenging. Existing ... -
Simultaneous manipulation and imaging of chemogenetically induced hydrogen peroxide in hardly transfectable endothelial cells
Eroğlu, Emrah (Sivas Cumhuriyet University, 2022)Hydrogen peroxide (H2O2) is a critical signaling molecule in vascular cells, which controls signaling events, yet it can cause pathological oxidative stress in excess. The lack of suitable tools undermined experimental ...